Effects of extracellular adenosine and ATP on cardiomyocytes

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Published by Landes in Austin .

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  • Heart cells.,
  • Adenosine -- Physiological effect.,
  • Adenosine triphosphate -- Physiological effect.,
  • Heart -- Physiology.,
  • Myocardium -- metabolism.,
  • Heart -- drug effects.,
  • Adenosine -- pharmacology.,
  • Adenosine -- metabolism.,
  • Adenosine Triphosphate -- pharmacology.,
  • Adenosine Triphosphate -- metabolism.

Edition Notes

Includes bibliographical references.

Book details

Statement[edited by] Amir Pelleg, Luiz Belardinelli.
SeriesMolecular biology intelligence unit ;, 6
ContributionsBelardinelli, Luiz.
LC ClassificationsQP114.C44 E44 1999
The Physical Object
Pagination221 p. :
Number of Pages221
ID Numbers
Open LibraryOL698908M
ISBN 101570595240
LC Control Number97046653

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Effects of Extracellular Adenosine and ATP on Cardiomyocytes [Pelleg, Amir, Belardinelli, Luiz] on *FREE* shipping on qualifying offers. Effects of Extracellular Adenosine and ATP on CardiomyocytesAuthor: Amir Pelleg. Additional Physical Format: Online version: Effects of extracellular adenosine and ATP on cardiomyocytes.

Austin: Landes, © (OCoLC)   Effects of Extracellular Adenosine and ATP on Cardiomyocytes 2 Medical books Effects of Extracellular Adenosine and ATP on Cardiomyocytes. Medical books. Medical Book Effects of Extracellular Adenosine and ATP on Cardiomyocytes 5 pages, 25 figures, 4. The effect of ATP was inhibited by a known purinergic P2-receptor antagonist, but not by a P1-receptor antagonist.

From these results, it is conceivable that the effect of ATP on Ca2+ efflux from cardiomyocytes is mediated through by: 4. Summary. Extracellular nucleotides such as ATP, or diadenosine polyphosphates and also adenosine play important receptor-mediated functions in the cardiovascular system by contributing to the regulation of vascular tone, myocardial contractility, and the function of blood by: The actions of ATP in the mammalian heart are to a large extent due to activation of A, and A 2 adenosine receptors by adenosine formed on degradation of this adenine nucleotide [2 3].

However, it has become increasingly clear that before its degradation to adenosine, extracellular ATP per se via activation of P 2 purinergic receptors (P 2-receptors) causes a positive inotropic by: 3. The effect of extracellular adenosine 5′-triphosphate (ATP) on Ca 2+ efflux from freshly isolated adult rat cardiomyocytes was examined.

Effects of extracellular adenosine and ATP on cardiomyocytes book ATP stimulated the efflux of 45 Ca 2+ from the cells in a concentration-dependent manner (–1 mM).Cited by: 1. Protective Effects of Extracellular ATP Are Mediated by P2 and Adenosine Receptors The role of P2 and P1 receptors involved in the anti-apoptotic effect of extracellular ATP was investigated using a panel of selective antagonists (Table 1).Cited by: 2.

Multifaceted Effects of Extracellular Adenosine Triphosphate and Adenosine in the Tumor–Host Interaction and Therapeutic Perspectives The Harvard community has made this article openly available.

Please share how this access benefits you. Your story matters Citation de Andrade Mello, Paola, Robson Coutinho-Silva, and Luiz Eduardo Baggio Savio. Adenosine has mainly anti-inflammatory effects by binding to the adenosine, or P1, receptors (A1, A2A, A2B, and A3).

These receptors are also expressed in many cells, including immune cells. The final effect of ATP and adenosine in immune responses depends on the fine regulatory balance between the 2 Cited by: Concentrations and references are quoted.

Figure 3. Anti-apoptotic effect of extracellular ATP mediated by both P2 and adenosine receptors (A). HUVECs, exposed to a simulated hypoxia during 2 hours, were treated with ATP 10 µM, P2 antagonist (10 µM suramin and PPADS), and adenosine antagonist (1 µM CGS).Cited by: 2.

Purinergic P2Y 2 receptors, G-protein coupled receptors that primarily couple with Gα q/proteins, are activated equipotently by adenosine-5′-triphosphate (ATP) and uridine-5′ce suggests that P2Y 2 agonists make potential drug candidates for the treatment of cardiovascular diseases.

However, selective non-nucleotide, small-molecule P2Y 2 agonists have yet to be Cited by: 4. Adenosine triphosphate (ATP) is released during neural stimulation and cardiac hypoxia and several mechanims of its action have been reported in different tissues.

ATP stimulates P1 and P2 purinergic receptors; it also activates receptor-operated channels and increases membrane permeability to small ions. In single rat ventricular cells under whole-cell patch-clamp, a stepwise application of Cited by: ATP (10 µM) significantly increased the migration of human cardiac fibroblasts after the 20 h incubation; this effect was reduced by the silencing of the P2X 4, P2X 7 and P2Y 2 receptors with siRNAs (n = 4, P Cited by: Many of the cells that “produce” adenosine also have receptors for adenosine.

Activation of these receptors often leads to an overall reduction of work performed and oxygen consumed by cells and organs. For example, adenosine causes a reduction of glomerular filtration, thereby reducing the transport work of the by: Abstract.

The effects of extracellular ATP on the L-type Ca 2+ current (I Ca), action potential, and resting and intracellular Ca 2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets.

Extracellular ATP decreased the peak amplitude of I Ca in a time- and concentration-dependent manner. The concentration-response relationship for ATP inhibition of I Ca Cited by: 1. Extracellular ATP acts as a local regulator of physiological functions in the cardiovascular system via P1 and P2 receptors.

However, little is known about the effect of ATP on the release of atrial natriuretic peptide (ANP) by: 2. Adenosine triphosphate (ATP) is actively released in the extracellular environment in response to tissue damage and cellular stress.

Through the activation of P2X and P2Y receptors, extracellular. Many endogenous signaling molecules have been described to induce protection against cardiac stress (hypoxic or ischemic stress). Among them, extracellular nucleotides such as adenosine tri-phosphate (ATP) and nucleosides, such as adenosines, are secreted by endothelial cells and cardiomyocytes during ischemia [].Purinergic signaling plays an important role in the cardiovascular system [8,9].Cited by: 2.

Introduction. Adenosine is the final product of the hydrolysis of extracellular adenine nucleotides such as ATP, ADP and AMP and has been suggested to be an important vasodilator in the cardiovascular system, exerting a cardioprotective action (Tucker and Linden, ).Cardiomyocytes and vascular cells readily form, transport and metabolize the endogenous adenosine, and at the Cited by: 6.

Growth inhibitory effect and apoptosis induced by extracellular ATP and adenosine on human gastric carcinoma cells: involvement of intracellular uptake of adenosine Ming-xia Wang 1 Cited by: In conclusion, adenosine originated from extracellular ATP hydrolysis favors normal collagen production by HSCF via A2A receptors.

Inhibition of unpredicted inosine formation by third party ADA cell providers (e.g., inflammatory cells) may be a novel therapeutic target to prevent inappropriate dermal remodeling via A3 receptors : Carina Herman-de-Sousa, Ana Rita Pinheiro, Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Maria Adeli.

Extracellular adenosine-5'-triphosphate (ATP) induces a number of cellular responses in plants and animals. In two books, Geoffrey Burnstock and Alexej Verkhratsky have aimed at drawing. In the in vivo analysis of extracellular ATP degradation by treating the heart with 10 μM ATP, we found that dialysate ATP concentrations ranged from to μM ( ± μM), and a ∼ times higher concentration of adenosine ( ± μM) was formed in the ISF at the same by: This gradient is used as an energy source by ATP synthase during the synthesis of an ATP molecule from a molecule of adenosine diphosphate (ADP) and an inorganic phosphate (Figure 1).

Without oxygen, oxidative phosphorylation stops: the proton gradient between the intermembrane space and the inner mitochondria is abolished, and ATP synthesis is Cited by: 3. Adenosine is a purine nucleoside that is produced by catabolism of adenosine triphosphate (ATP) by many different cell types.

result in a rapid increase in extracellular levels of adenosine. This in turn results in activation of local adenosine receptors on cardiomyocytes and vascular endothelial cells which mediate a reduction in metabolic Author: A. Dana, D.

Yellon, G. Baxter. Extracellular adenosine 5′-triphosphate (ATP) exerts various potent actions in the cardiovascular system, e.g. regulation of vascular tone and platelet aggregation []. In the cardiomyocyte, ATP has been shown to cause a pronounced positive inotropic effect and may also act in synergy with β-adrenergic agonists to augment myocyte contractility [ 3, 6 - 9 ].Cited by: Abstract Cerebral ischaemia rapidly depletes cellular ATP.

Whilst this deprives brain tissue of a valuable energy source, the concomitant production of adenosine mitigates the damaging effects of e Cited by: Extracellular ATP is known to augment cardiac contractility by increasing intracellular Ca 2+ concentration ([Ca 2+] i) in cardiomyocytes; however, the status of ATP-mediated Ca 2+ mobilization in hearts undergoing ischemia-reperfusion (I/R) has not been examined previously.

In this study, therefore, isolated rat hearts were subjected to 10–30 min of global ischemia and 30 min of reperfusion Cited by: Adenosine is derived from the breakdown of extra- or intracellular ATP and is released upon a variety of physiological and pathological stimuli from neuronal and non-neuronal sources, i.e.

from glial cells and exerts effects diffusing far away from release by:   Adenosine Uptake is the Major Effector of Extracellular ATP Toxicity in Human Cervical Cancer Cells Article (PDF Available) in Molecular Biology of the. Adenosine is an organic compound that occurs widely in nature in the form of diverse derivatives.

The molecule consists of an adenine, minus one H atom, attached to a ribose, minus one OH group, via a β-N 9-glycosidic ine is one of four nucleoside building blocks to RNA, which is essential for all derivatives include the energy carriers adenosine mono- di- and a: C₁₀H₁₃N₅O₄.

ATP acting via different subtypes of P2X and P2Y receptors induces contractions or relaxation of mammalian smooth muscles, while in skeletal muscles, ATP can pre- and postsynaptically modulate effect of : Ayrat U. Ziganshin, Sergey N.

Grishin. In addition, ectonucleotidases generate extracellular adenosine, which abrogates nucleotide-mediated effects and activates adenosine receptors, often with opposing (patho-) physiological effects. Adenosine generation. The first step in the production of adenosine involves the conversion of ATP/ADP to AMP.

It is carried out by ENTPD1, also known. Imatinib and dasatinib had moderate effects on both OCR and ATP content; predominantly observed at µM (Figs.

10C and D, respectively). Nilotinib and lapatinib did not have any major effect on either OCR or ATP content of the cells (Figs. 10E and F, respectively). Similar effects were observed when iPSC-derived cardiomyocytes were maintained Cited by: Role of the Extracellular Ca2+/cyclic AMP-Adenosine Signaling Pathways in Cardioprotection.

effect was inhibited by the A(1) adenosine receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine. Adenosine triphosphate (ATP) is an organic compound that provides energy to drive many processes in living cells, e.g. muscle contraction, nerve impulse propagation, and chemical in all forms of life, ATP is often referred to as the "molecular unit of currency" of intracellular energy transfer.

When consumed in metabolic processes, it converts either to adenosine diphosphate Chemical formula: C₁₀H₁₆N₅O₁₃P₃. Extracellular ATP acts as a potent agonist on cardiomyocytes, inducing a broad range of physiological responses via P2 purinoceptors.

Its concentration in the interstitial space within the heart. The extracellular ATP pathway is another source of renal adenosine (for reviews, see Refs. 58 and ). This pathway is not dependent on an imbalance between energy supply and energy demand and is consequently a source of extracellular adenosine regardless of the metabolic status of the by: E-Mail Address.

Password. Forgotten Password. Remember Me. The answer lies with an energy-supplying molecule called adenosine triphosphate, or ATP. ATP is a small, relatively simple molecule (Figure ), but within some of its bonds, it contains the potential for a quick burst of energy that can be harnessed to perform cellular work.

Adenosine is an endogenous purine formed by adenine and D-ribose produced from the degradation of ATP, ADP and AMP by ectoenzymes (predominantly CD39 and CD73) which is a process universal to most mammalian cells.

11 The production of adenosine is widely employed in cellular signalling as an indication of ATP turnover.

Extracellular adenosine Author: Andrew Grace, Gareth Dk Matthews. Adenosine has a short half-life in the blood (a few seconds) and in spinal cerebrospinal fluid (10 to 20 minutes) (Antonioli).

Adenosine is released from within the cell after production of ATP by mitochondria inside the cell (intracellular space) and then passes through the cell wall into the spaces between cells (extracellular space).

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